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KMID : 1025520050470020187
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Journal of Animal Science and Technology
2005 Volume.47 No. 2 p.187 ~ p.194
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The Amount of Telomeres and Telomerase Activity on Chicken Embryonic Cells During Developmental Stages
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Cho E. J.
Choi C. H.
Sohn S. H.
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Abstract
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Telomeres locate at the end of chromosomes and consist of a tandem repeat sequence of (TTAGGG)n and associated proteins. Telomerase is a ribonucleoprotein which act as a template for the synthesis of telomeric DNA. Telomeres are essential for chromosome stability and are related with cell senescence, apoptosis and cancer. This study was carried out to analyze the amount of telomeres and telomerase activity of chicken cells during embryonic and developmental stages. The whole embryos and prenatal tissues such as brain, heart, liver, kidney and testis at different developmental stages were obtained from Korean Native Chicken. The amount of telomeres on embryonic cells was analyzed by quantitative fluorescence in situ hybridization (Q-FISH) techniques using the chicken telomeric DNA probe. Telomerase activity was measured by telomeric repeat amplification protocol (TRAP) assay. Results indicated that the amounts of telomeric DNA on the most embryonic cells were gradually decreased during ontogenesis. Furthermore, the quantity of telomeres was quite different among embryonic tissues according to developmental origin. The relative amount of telomeres has more in regenerative cells such as embryonic disc and testicular cells than in non-regenerative cells such as liver, brain, heart and kidney cells. Telomerase activity was also highly detectable in most chicken cells at early embryonic stages. After 9 days of incubation, however, the telomerase activitie was gradually decreased in brain, heart and liver cells, but not in the testicular and kidney cells. In conclusion, the amount of telomeres and telomerase activity are closely related to cell differentiation and tissue specificity during chicken developmental stages. Moreover, the highly positive correlation is existed between telomere amount and telomerase activity in chicken cells.
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KEYWORD
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Chicken, Embryonic tissues, Telomere, Telomerase, Q-FISH, TRAP
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